منابع مشابه
Principle and applications of digital PCR.
Digital PCR represents an example of the power of PCR and provides unprecedented opportunities for molecular genetic analysis in cancer. The technique is to amplify a single DNA template from minimally diluted samples, therefore generating amplicons that are exclusively derived from one template and can be detected with different fluorophores or sequencing to discriminate different alleles (e.g...
متن کاملContext-aware systems: concept, functions and applications in digital libraries
Background and Aim Among the places that context-aware systems and services would be very useful, are libraries. The purpose of this study is to achieve a coherent definition of context aware systems and applications, especially in digital libraries. Method: This was a review article that was conducted by using Library method by searching articles and e-books on websites and databases. Results:...
متن کاملDigital PCR.
The identification of predefined mutations expected to be present in a minor fraction of a cell population is important for a variety of basic research and clinical applications. Here, we describe an approach for transforming the exponential, analog nature of the PCR into a linear, digital signal suitable for this purpose. Single molecules are isolated by dilution and individually amplified by ...
متن کاملDigital PCR
We are very pleased to be able to bring you this special issue of iomolecular Detection and Quantification which focuses on digital CR (dPCR). The underpinning method of dPCR, which was coined n 1999 [1], actually predates qPCR [2] and is a powerful technique hat could offer improved sensitivity, precision and reproducibility 3]. Now is an exciting time for this method and there are numerous xa...
متن کاملExpression-PCR (E-PCR): overview and applications.
1Department of Immunology, Waiter Reed Army Institute of Research, Washington, D.C. 20307-5100; 2Tropical Disease Unit, Division of Infectious Diseases, The Toronto Hospital and The University of Toronto, Toronto, Ontario, Canada M5G 2C4 In vitro transcription and translation are powerful tools for examining the s t ructure-funct ion relationships of proteins. Genes can be cloned into plasmid v...
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ژورنال
عنوان ژورنال: Turkish Bulletin of Hygiene and Experimental Biology
سال: 2016
ISSN: 0377-9777,1308-2523
DOI: 10.5505/turkhijyen.2016.48902